phospho 284 acc (Cell Signaling Technology Inc)
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Phospho 284 Acc, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 717 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phospho+acc+ser79/Phospho-Acetyl-CoA+Carboxylase+(Ser79)+Rabbit+mAb/pm41932650-133-4-27
Average 96 stars, based on 717 article reviews
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Blocking Assay:Article Title: d -Allulose Ameliorates Fructose-Induced Skeletal Muscle Insulin Resistance via Regulation of Ectopic Lipid Accumulation Independent of Body Weight Changes Article Snippet: .. Following the blocking step, the membranes were incubated for 1 h at room temperature with the following primary antibodies: Phospho-Akt (Ser473) #9271, AMP-activated protein kinase (AMPK)α #2532, Phospho-AMPKα (Thr172) #2531, acetyl-CoA carboxylase (ACC; C83B10) # Article Title: Preventing and correcting polycystic ovary syndrome by targeting anti-Müllerian hormone signaling in minipuberty and adulthood in mice. Article Snippet: Insulin response measurement Basal blood glucose levels were measured after a 6-h fast for the ITT before i.p. insulin administration (0.5 UI kg–1 body weight, Eli Lilly and Company, catalogue n HI0210) and 15, 30, 45, 60, 90 and 120 min after glucose administration using a Accu-Chek Guide glucometer. .. Tissues, all dissected during the diestrous stage, were homogenized using Parcellys 24 touch homogenizer (Bertin Technologies) in cold RIPA buffer (200 mM Tris/HCl pH 7.4, 130 mM NaCl, 10% (v/v) glycerol, 0.1% (v/v) SDS, 1% (v/v) Triton X-100, 10 mM MgCl2) with anti-proteases and anti-phosphatases (Sigma-Aldrich), and lysates were centrifuged for 30min at 18,000g and 4 C. Liver, white adipose tissue (intra-abdominal adipose tissue), ovaries and pancreas total protein lysates were separated on SDS–polyacrylamide gel electrophoresis (SDS–PAGE), electrotransferred to polyvinylidene difluoride membranes and probed successively with the following antibodies: hormone-sensitive lipase/HSL (1:1,000; Abcam, catalogue n ab45422); phospho-HSL (Ser660) (1:1000; Cell Signaling Technology, catalogue no. 4126); ACC (1:1000; Millipore, catalogue n 04-322); Incubation:Article Title: d -Allulose Ameliorates Fructose-Induced Skeletal Muscle Insulin Resistance via Regulation of Ectopic Lipid Accumulation Independent of Body Weight Changes Article Snippet: .. Following the blocking step, the membranes were incubated for 1 h at room temperature with the following primary antibodies: Phospho-Akt (Ser473) #9271, AMP-activated protein kinase (AMPK)α #2532, Phospho-AMPKα (Thr172) #2531, acetyl-CoA carboxylase (ACC; C83B10) # Article Title: Preventing and correcting polycystic ovary syndrome by targeting anti-Müllerian hormone signaling in minipuberty and adulthood in mice. Article Snippet: Insulin response measurement Basal blood glucose levels were measured after a 6-h fast for the ITT before i.p. insulin administration (0.5 UI kg–1 body weight, Eli Lilly and Company, catalogue n HI0210) and 15, 30, 45, 60, 90 and 120 min after glucose administration using a Accu-Chek Guide glucometer. .. Tissues, all dissected during the diestrous stage, were homogenized using Parcellys 24 touch homogenizer (Bertin Technologies) in cold RIPA buffer (200 mM Tris/HCl pH 7.4, 130 mM NaCl, 10% (v/v) glycerol, 0.1% (v/v) SDS, 1% (v/v) Triton X-100, 10 mM MgCl2) with anti-proteases and anti-phosphatases (Sigma-Aldrich), and lysates were centrifuged for 30min at 18,000g and 4 C. Liver, white adipose tissue (intra-abdominal adipose tissue), ovaries and pancreas total protein lysates were separated on SDS–polyacrylamide gel electrophoresis (SDS–PAGE), electrotransferred to polyvinylidene difluoride membranes and probed successively with the following antibodies: hormone-sensitive lipase/HSL (1:1,000; Abcam, catalogue n ab45422); phospho-HSL (Ser660) (1:1000; Cell Signaling Technology, catalogue no. 4126); ACC (1:1000; Millipore, catalogue n 04-322); Article Title: Timing of glucose intake drives distinct hepatic outcomes: Divergent glucose and lipid metabolism. Article Snippet: Objective: Excessive sugar intake is strongly associated with insulin resistance and type 2 diabetes (T2D), yet the metabolic consequences of nutrient timing—specifically glucose consumption during fasting versus feeding—remain poorly understood.. Methods: C57BL/6 mice were subjected to an every-other-day fasting (EODF) regimen and randomly divided into three groups: control (tap water only), food-glucose (FG; glucose water during feeding), and starvation-glucose (SG; glucose water during fasting).. After 22 weeks, metabolic phenotypes, hepatic lipid profiles, insulin signaling markers, and hepatic transcriptomes were analyzed. other:Article Title: Acetyl-CoA carboxylase obstructs CD8 + T cell lipid utilization in the tumor microenvironment Article Snippet: Article Title: Acetyl-CoA carboxylase obstructs CD8 + T cell lipid utilization in the tumor microenvironment Article Snippet: Nucleic Acid Electrophoresis:Article Title: Preventing and correcting polycystic ovary syndrome by targeting anti-Müllerian hormone signaling in minipuberty and adulthood in mice. Article Snippet: Insulin response measurement Basal blood glucose levels were measured after a 6-h fast for the ITT before i.p. insulin administration (0.5 UI kg–1 body weight, Eli Lilly and Company, catalogue n HI0210) and 15, 30, 45, 60, 90 and 120 min after glucose administration using a Accu-Chek Guide glucometer. .. Tissues, all dissected during the diestrous stage, were homogenized using Parcellys 24 touch homogenizer (Bertin Technologies) in cold RIPA buffer (200 mM Tris/HCl pH 7.4, 130 mM NaCl, 10% (v/v) glycerol, 0.1% (v/v) SDS, 1% (v/v) Triton X-100, 10 mM MgCl2) with anti-proteases and anti-phosphatases (Sigma-Aldrich), and lysates were centrifuged for 30min at 18,000g and 4 C. Liver, white adipose tissue (intra-abdominal adipose tissue), ovaries and pancreas total protein lysates were separated on SDS–polyacrylamide gel electrophoresis (SDS–PAGE), electrotransferred to polyvinylidene difluoride membranes and probed successively with the following antibodies: hormone-sensitive lipase/HSL (1:1,000; Abcam, catalogue n ab45422); phospho-HSL (Ser660) (1:1000; Cell Signaling Technology, catalogue no. 4126); ACC (1:1000; Millipore, catalogue n 04-322); Pyrolysis Gas Chromatography:Article Title: 1,1-Diethoxyethane enhances aerobic respiration in human mitochondria via activation of AMP-activated protein kinase. Article Snippet: Mitotracker® Red CMXRos (#9082) and protease/phosphatase inhibitor cocktail (100X; #5872) were obtained from Cell Signaling Technology (Danvers, MA, USA). .. The following antibodies were used: AMPK (1:1000; #2532), phospho-AMPK (Thr172) (1:1000; #2535), ACC (1:1000; #3676), Article Title: 1,1-Diethoxyethane enhances aerobic respiration in human mitochondria via activation of AMP-activated protein kinase Article Snippet: Mitotracker® Red CMXRos (#9082) and protease/phosphatase inhibitor cocktail (100X; #5872) were obtained from Cell Signaling Technology (Danvers, MA, USA). .. The following antibodies were used: AMPK (1:1000; #2532), phospho-AMPK (Thr172) (1:1000; #2535), ACC (1:1000; #3676), |
